Evaluation of daily germination in two rice cultivars using linear regression
Article Sidebar
Main Article Content
Resumo
Among the types of plant seed priming, hydropriming stands out with proven benefits in germination properties and is essential for standardizing germination in rice.The objective was to evaluate the daily dynamics of hydroconditioned germinated seeds of rice cultivars INCA P-5 and IACuba-41 (Oryza sativa L.) using a simple linear regression model. In January 2024, in Río Cauto, Granma, Cuba, the experiment was conducted with 200 rice seeds (100 per cultivar: INCA LP-5 and IACuba-41). The seeds, selected without defects and with moisture adjusted to 9-10%, were imbibed for 72 hours with irrigation water, dried for 48 hours in the shade, and sown in trays with local Vertisol soil. The number of germinated seeds was evaluated daily for 14 days. A simple linear regression model was applied (days as independent variable, germination as dependent) using ordinary least squares, with the slope (a), intercept (b) and their bootstrap confidence intervals with 1,999 repetitions and 95,0 percentile. Heteroscedasticity, autocorrelation of residuals, predicted values and their standard errors were also determined. The results confirm that the germinated seeds per day in both cultivars do not fit a linear regression model with asymmetric stages of dormancy breaking, germination peak and subsequent decline, with a slight lower dispersion or a more concentrated germination of the INCA LP-5 cultivar compared to IACuba-41
Article Details
Este trabalho encontra-se publicado com a Creative Commons Atribuição-NãoComercial 4.0.
Aquellos autores/as que tengan publicaciones con esta revista, aceptan los términos siguientes:
- Los autores/as conservarán sus derechos de autor y garantizarán a la revista el derecho de primera publicación de su obra, el cuál estará simultáneamente sujeto a la Licencia Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) que permite a terceros compartir la obra siempre que se indique su autor y su primera publicación esta revista. Bajo esta licencia el autor será libre de:
- Compartir — copiar y redistribuir el material en cualquier medio o formato
- Adaptar — remezclar, transformar y crear a partir del material
- El licenciador no puede revocar estas libertades mientras cumpla con los términos de la licencia
Bajo las siguientes condiciones:
- Reconocimiento — Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace.
- NoComercial — No puede utilizar el material para una finalidad comercial.
- No hay restricciones adicionales — No puede aplicar términos legales o medidas tecnológicas que legalmente restrinjan realizar aquello que la licencia permite.
- Los autores/as podrán adoptar otros acuerdos de licencia no exclusiva de distribución de la versión de la obra publicada (p. ej.: depositarla en un archivo telemático institucional o publicarla en un volumen monográfico) siempre que se indique la publicación inicial en esta revista.
- Se permite y recomienda a los autores/as difundir su obra a través de Internet (p. ej.: en archivos telemáticos institucionales o en su página web) antes y durante el proceso de envío, lo cual puede producir intercambios interesantes y aumentar las citas de la obra publicada. (Véase El efecto del acceso abierto).
Referências
AHRENS, W.H.; COX, D.J.; BUDHWAR, G.: “Use of the arcsine and square root transformations for subjectively determined percentage data”, Weed Science, 38(4-5): 452-458, 1990, ISSN: 0043-1745, DOI: https://doi.org/10.1017/s0043174500056824.
ALI, U.; ULLAH, S.: “Hydrothermal time model analysis of seed germination responses to osmotic stress and temperatures in Cicer arietinum L. varieties”, Journal of Xi’an Shiyou University, Natural Science Edition, 18(12): 1279-1287, 2022, ISSN: 1673-064X.
ANSARI, O.; GHADERIFAR, F.; ZADEH, F.; MORADI, A.: “Evaluation of nonlinear regression models to describe seed germination response of mountain rye (Secale mountanum) to temperature.”, 2017, DOI: https://doi.org/10.29252/yujs.3.2.31.
BARIK, S.R.; PANDIT, E.; SANGHAMITRA, P.; MOHANTY, S.P.; BEHERA, A.; MISHRA, J.; NAYAK, D.K.; BASTIA, R.; MOHARANA, A.; SAHOO, A.: “Unraveling the genomic regions controlling the seed vigour index, root growth parameters and germination per cent in rice”, PLoS One, 17(7): e0267303, 2022, ISSN: 1932-6203, DOI: https://doi.org/10.1371/journal.pone.0267303.
BEWLEY, J.D.; BRADFORD, K.; HILHORST, H.: Seeds: physiology of development, germination and dormancy, Ed. Springer Science & Business Media, 2012, ISBN: 1-4614-4692-9.
BOLKER, B.M.; BROOKS, M.E.; CLARK, C.J.; GEANGE, S.W.; POULSEN, J.R.; STEVENS, M.H.H.; WHITE, J.-S.S.: “Generalized linear mixed models: a practical guide for ecology and evolution”, Trends in ecology & evolution, 24(3): 127-135, 2009, ISSN: 0169-5347, DOI: https://doi.org/10.1016/j.tree.2008.10.008.
BRADFORD, K.J.: “Applications of hydrothermal time to quantifying and modeling seed germination and dormancy”, Weed Science, 50(2): 248-260, 2002, ISSN: 0043-1745, DOI: https://doi.org/10.1614/0043-1745(2002)050[0248:AOHTTQ]2.0.CO;2.
CARVALHO, F.J.; SANTANA, D.G. de; ARAÚJO, L.B. de: “Why analyze germination experiments using Generalized Linear Models?”, Journal of Seed Science, 40(3): 281-287, 2018, ISSN: 2317-1537, DOI: https://doi.org/10.1590/2317-1545v40n3185259.
CATIEMPO, R.L.; PHOTCHANACHAI, S.; POWELL, A.F.; STRICKLER, S.R.; WONGS‐AREE, C.: “Transcriptome analysis suggests the role of expansin genes in the improved germination of sunflower (Helianthus annuus L.) seeds after hydropriming”, Crop Science, 64(3): 1862-1873, 2024, ISSN: 0011-183X, DOI: http://dx.doi.org/10.1002/csc2.21221.
CHENG, E.; SONG, P.; WANG, B.; HOU, T.; WU, L.; ZHANG, B.: “Determination of rice seed vigor by low-field nuclear magnetic resonance coupled with machine learning.”, 2022, DOI: https://doi.org/10.35633/inmateh-67-52.
CHOI, J.-Y.; JU, Y.-H.; NAKAMICHI, A.; CHO, S.-W.; WOO, S.-H.; SAKAGAMI, J.-I.: “Effect of seed hydropriming on the elongation of plumule and radicle during the germination process and changes in enzyme activity under water-deficient conditions”, Plants, 13(24): 3537, 2024, ISSN: 2223-7747, DOI: https://doi.org/10.3390/plants13243537.
COVELL, S.; ELLIS, R.; ROBERTS, E.; SUMMERFIELD, R.: “The influence of temperature on seed germination rate in grain legumes: I. A comparison of chickpea, lentil, soyabean and cowpea at constant temperatures”, Journal of Experimental Botany, 37(5): 705-715, 1986, ISSN: 1460-2431, DOI: . https://doi.org/10.1093/jxb/37.5.705.
HAY, F.R.; MEAD, A.; BLOOMBERG, M.: “Modelling seed germination in response to continuous variables: use and limitations of probit analysis and alternative approaches”, Seed Science Research, 24(3): 165-186, 2014, ISSN: 0960-2585, DOI: https://doi.org/10.1017/S096025851400021X.
HERNÁNDEZ-JIMÉNEZ, A.; PÉREZ-JIMÉNEZ, J.M.; BOSCH-INFANTE, D.; SPECK, N.C.: “La clasificación de suelos de Cuba: énfasis en la versión de 2015”, Cultivos tropicales, 40(1), 2019, ISSN: 0258-5936.
ISTA RULES: International rules for seed testing, [en línea], Inst. International Seed Testing Association, 2022, Disponible en: https://seedhealth.org/files/2022/03/ISTA_Rules_2022_SHMethods_7-004.pdf.
ISWAHYUDI, I.; WIDODO, W.; WARKOYO, W.; SUTANTO, A.; GARFANSA, M.P.; MUJIYANTI, W.A.; SHOLEH, M.S.: “Investigating the impact of microplastics type of polyethylene, polypropylene, and polystyrene on seed germination and early growth of rice plants”, Environmental Quality Management, 34(1): e22287, 2024, ISSN: 1088-1913, DOI: https://doi.org/10.1002/tqem.22287.
JIANG, M.; XU, L.; CHEN, X.; ZHU, H.; FAN, H.: “Soil quality assessment based on a minimum data set: a case study of a county in the typical river delta wetlands”, Sustainability, 12(21): 9033, 2020, ISSN: 2071-1050, DOI: https://doi.org/10.3390/su12219033.
KHALEQUZZAMAN; ULLAH, H.; HIMANSHU, S.K.; ISLAM, N.-E.-T.; TISARUM, R.; CHA-UM, S.; DATTA, A.: “Seed priming improves germination, yield, and water productivity of cotton under drought stress”, Journal of Soil Science and Plant Nutrition, 23(2): 2418-2432, 2023, ISSN: 0718-9508, DOI: https://doi.org/10.1007/s42729-023-01196-5.
KHARB, V.; SHARMA, V.; DHALIWAL, S.S.; KALIA, A.: “Influence of iron seed priming on seed germination, growth and iron content in rice seedlings”, Journal of Plant Nutrition, 46(16): 4054-4062, 2023, ISSN: 0190-4167, DOI: https://doi.org/1080/01904167.2023.2220731.
KIANI, S.; ESHRAGHI-NEJAD, M.; ESMAEILZADE-MORIDANI, M.; HOSSAIN, M.: “Regression analysis of salinity, hydropriming and their interaction effect on Fennel (Foeniculum vulgare) seeds germination”, International Journal of Traditional and Herbal Medicine, 1(5): 130-135, 2013.
KUMAR, P.; THAKUR, N.: “Examination of seed germination process and assess phytotoxicity in Vigna radiata (Mung bean) and Allium cepa (Onion) using triple-doped green hematite nanoparticles and their photocatalytic potential”, Environmental Science and Pollution Research, : 1-17, 2025, ISSN: 1614-7499, DOI: https://doi.org/10.1007/s11356-025-36735-3.
LIU, C.; LI, C.; BING, H.; ZHAO, J.; LI, L.; SUN, P.; LI, T.; DU, D.; ZHAO, J.; WANG, X.: “Integrated physiological, transcriptomic, and metabolomic analysis reveals the mechanism of guvermectin promoting seed germination in direct-seeded rice under chilling stress”, Journal of Agricultural and Food Chemistry, 71(19): 7348-7358, 2023, ISSN: 0021-8561, DOI: . https://doi.org/10.1021/acs.jafc.3c00559.
MAMANI, G.Q.; DUARTE, M.L.; ALMEIDA, L.S. de; MARTINS FILHO, S.: “Non-parametric survival analysis in seed germination of forest species”, Journal of Seed Science, 46, 2024, ISSN: 2317-1537, DOI: https://doi.org/10.1590/2317-1545v46288345.
MARA, M.N.; HIDAYAT, S.S.; PUTRI, F.T.; RAHMAWATI, D.; WAHYUNI, S.; PRABOWO, M.C.A.; KABIR, N.N.S.; INDRA, R.T.: “Overcoming imbalanced rice seed germination classification: Enhancing accuracy for effective seedling identification”, IAES International Journal of Artificial Intelligence (IJ-AI), 14(1): 62-72, 2025, ISSN: 2089-4872, DOI: https://doi.org/10.11591/ijai.v14.i1.pp62-72.
MCNAIR, J.N.; SUNKARA, A.; FROBISH, D.: “How to analyse seed germination data using statistical time-to-event analysis: non-parametric and semi-parametric methods”, Seed Science Research, 22(2): 77-95, 2012, ISSN: 1475-2735, DOI: https://doi.org/10.1017/S0960258511000547.
MESGARAN, M.; MASHHADI, H.; ALIZADEH, H.; HUNT, J.; YOUNG, K.; COUSENS, R.: “Importance of distribution function selection for hydrothermal time models of seed germination”, Weed Research, 53(2): 89-101, 2013, ISSN: 0043-1737, DOI: https://doi.org/10.1111/wre.12008.
MONDO, V.V.; NASCENTE, A.; NEVES, P. de C.; TAILLEBOIS, J.; OLIVEIRA, F.: “Seed hydropriming in upland rice improves germination and seed vigor and has no effects on crop cycle and grain yield.”, 2016, DOI: https://doi.org/10.21475/ajcs.2016.10.11.PNE70.
PEDROSO, R.M.; NETO, D.D.; VICTORIA FILHO, R.; FISCHER, A.J.; AL-KHATIB, K.: “Modeling germination of smallflower umbrella sedge (Cyperus difformis L.) seeds from rice fields in California across suboptimal temperatures”, Weed Technology, 33(5): 733-738, 2019, ISSN: 0890-037X, DOI: https://doi.org/10.1017/wet.2019.52.
PŁAŻEK, A.; DUBERT, F.; KOPEĆ, P.; DZIURKA, M.; KALANDYK, A.; PASTUSZAK, J.; WOLKO, B.: “Seed hydropriming and smoke water significantly improve low-temperature germination of Lupinus angustifolius L.”, International Journal of Molecular Sciences, 19(4): 992, 2018, ISSN: 1422-0067, DOI: https://doi.org/10.3390/ijms19040992.
PUTEH, A.B.; ROSLI, R.; MOHAMAD, R.B.: “Dormancy and cardinal temperatures during seed germination of five weedy rice (Oryza spp.) strains”, Pertanika Journal of Tropical Agricultural Science, 33(2): 243-250, 2010, ISSN: 1511-3701.
R CORE TEAM: “R: A language and environment for statistical computing”, R foundation for statistical computing, Vienna, Austria, 2021.
RANMEECHAI, N.; LACAP, A.T.; TAC-AN, M.I.A.; BAYOGAN, E.R.V.: “Seed germination and vigor of four Philippine rice varieties as influenced by hydropriming and storage at various durations”, Philippine Journal of Science, 151(2): 755-765, 2022, ISSN: 0031-7683, DOI: https://doi.org/10.56899/151.02.18.
REN, M.; TAN, B.; XU, J.; YANG, Z.; ZHENG, H.; TANG, Q.; ZHANG, X.; WANG, W.: “Priming methods affected deterioration speed of primed rice seeds by regulating reactive oxygen species accumulation, seed respiration and starch degradation”, Frontiers in Plant Science, 14: 1267103, 2023, ISSN: 1664-462X, DOI: https://doi.org/10.3389/fpls.2023.1267103.
SAIRDAMA, S.S.; MATAKENA, S.: “Factors Affecting Production Rice Farming (Oryza sativa. L) in Biha Village Makimi District, Nabire Regency, Indonesia”, Jurnal Penelitian Pendidikan IPA, 11(5): 43-50, 2025, ISSN: 2407-795X, DOI: https://doi.org/10.29303/jppipa.v11i5.10884.
SALES, E.; VIRUEL, J.; DOMINGO, C.; MARQUÉS, L.: “Genome wide association analysis of cold tolerance at germination in temperate japonica rice (Oryza sativa L.) varieties”, PloS one, 12(8): e0183416, 2017, ISSN: 1932-6203, DOI: https://doi.org/10.1371/journal.pone.0183416.
SCOTT, S.; JONES, R.: “Low temperature seed germination of Lycopersicon species evaluated by survival analysis”, Euphytica, 31(3): 869-883, 1982, ISSN: 0014-2336, DOI: https://doi.org/10.1007/BF00039227.
SCOTT, S.J.; JONES, R.; WILLIAMS, Wa.: “Review of data analysis methods for seed germination 1”, Crop science, 24(6): 1192-1199, 1984, ISSN: 0011-183X, DOI: https://doi.org/10.2135/cropsci1984.0011183X002400060043x.
SILESHI, G.W.: “A critique of current trends in the statistical analysis of seed germination and viability data”, Seed Science Research, 22(3): 145-159, 2012, ISSN: 1475-2735, DOI: https://doi.org/10.1017/S0960258512000025.
THERNEAU, T.A.: Package for Survival Analysis in R. R package version 3.7-0, [en línea], 2024, Disponible en: https://CRAN.R-project.org/package=survival.
WATT, M.S.; BLOOMBERG, M.; FINCH, W.E.: “Development of a hydrothermal time model that accurately characterizes how thermoinhibition regulates seed germination”, Plant, Cell & Environment, 34(5): 870-876, 2011, ISSN: 0140-7791., DOI: https://doi:10.1111/j.1365-3040.2011.02292.x.
ZHANG, Z.; XUE, J.; GU, T.; WANG, H.; CHAUHAN, B.S.: “Effect of environmental factors on seed germination and seedling emergence of weedy rice (Oryza sativa f. spontanea) in China”, Weed Science, 72(1): 54-60, 2024, ISSN: 0043-1745, DOI: . https://doi:10.1017/wsc.2024.59.