Study of Enzymes Pre-Treatments in Biomasses Dedicated to Biogas Production

Main Article Content

Carlos M. Martínez-Hernández

Abstract

This work presents a study of the influence of an enzyme pre-treatment application to different substrates utilized for biogas production. The substrates studied were biomass of agricultural origin (sorghum-R-132, sunflower JE-94, corn and dasheen) and a tavern residual (white bread). The pre-treatment consisted on the addition of one enzyme (ZY maXX XL 200) in dose of 100 μL/syringes per 350 mg substrates diluted in 30 mL of inoculum. Substrates underwent a biodigestion process and the results were compared with those obtained in absence of the enzyme pre-treatment. Increments were achieved in the maximum specific biogas yield in the substrates. The best results took place with Colocacia esculenta (1918,07 LCH4/kgSV); bread (1792,81 LCH4/kgSV); sunflower JE-94 (1676, 06 LCH4/kgSV); sorghum R-132 (1145,78 LCH4/kgSV) and corn (1139,03 LCH4/kgSV) being obtained more than 100% increment in the maximum specific biogas yield when the treatment is used with enzymes and pig inoculum. On the other hand, it was observed that pH evolves in a similar way in substrates evaluated with enzyme treatment and without pre-treatment.

Article Details

How to Cite
Martínez-Hernández, C. M. (2017). Study of Enzymes Pre-Treatments in Biomasses Dedicated to Biogas Production. Revista Ciencias Técnicas Agropecuarias, 26(2), 55–64. Retrieved from https://revistas.unah.edu.cu/index.php/rcta/article/view/491
Section
Original Articles
Author Biography

Carlos M. Martínez-Hernández, Universidad Central “Marta Abreu”de las Villas

Dr.C., Prof. Titular.

References

BRULE M.: The effect of enzyme additives on the anaerobic digestion of energy crops, University of Hohenheim, Ph.D. Thesis, Germany,

p., 2014.

BRULE, M.; VOGTHEER, J.; LEMMER, A.; OECHSNER, H.; JUNGBLUTH, T.: “Effect of enzyme addition on the methane yields of effluents

from a full scale biogas plant”, Landtechnik, 66(1): 50-52, 2011, ISSN 0023-8082.

KURAKAKE, M.; IDE, N.; KOMAKI, T.: “Biological Pretreatment with Two Bacterial Strains for Enzymatic Hydrolysis of Office Paper”,

Current Microbiology, 54(6): 424-428, 2007, ISSN: 0343-8651, 1432-0991, DOI: http://dx.doi.org/10.1007/s00284-006-0568-6.

MARTÍNEZ, C.; OECHSNER, H.; BRULÉ, M.; MARAÑON, E.: “Estudio de algunas propiedades físico-mecánicas y químicas de residuos

orgánicos a utilizar en la producción de biogás en Cuba”. Revista Ciencias Técnicas Agropecuarias, 23(2): 63-69, 2014, ISSN: 1010-2760,

-0054.

MARTÍNEZ, C.; OECHSNER, H.; REINHARDT, A.; GARCÍA, Y.; LÓPEZ, L.: “Studies of chemical-thermal pre-treatment in biomass to use

for biogas production in Cuba”. Journal of Basic and Applied Research International, 14(3): 215-224, 2015, ISSN: 2395-3438, 2395-3446.

MARTÍNEZ, C.; GARCÍA, Y.: “Utilización de pre-tratamientos básicos y específicos para la producción de biogás. Revisión y análisis”,

Revista Ciencias Técnicas Agropecuarias, 25(3): 81-92, 2016, ISSN: 2071-0054, DOI: http://dx.doi.org/10.13140/RG.2.2.34865.99688.

OFICINA NACIONAL DE NORMALIZACIÓN: Determinación de la humedad inicial y de la materia seca, no. NC 74-22, 1985.

OFICINA NACIONAL DE NORMALIZACIÓN: Determinación del contenido de cenizas, no. NC 74-30, 1985.

TAHERZADEH, M.J.; KARIMI, K.: “Pretreatment of lignocellulosic wastes to improve ethanol and biogas production. A review”, International

Journal of Molecular Science, 9(9): 1621-1651, 2008, ISSN: 1422-0067, DOI: http://dx.doi.org/10.3390/ijms9091621.

TANIGUCHI, M.; SUSUKI, H.; WATANABE, D.; SAKAI, K.; HOSHINO, K.; TANAKA, T.: “Evaluation of pretreatment with Pleurotus

ostreatus for enzymatic hydrolysis of rice straw”, Journal of Bioscience and Bioengineering, 100(6): 637-643, 2005, ISSN: 1389-1723,

DOI: http://dx.doi.org/10.1263/jbb.100.637.

VINTILOUI, A.; BRULÉ, M.; LEMMER. A.; OECHSNER, H.; JUNGBLUTH, T.: “Influence of temperature and pH on enzyme activity in

the biogas process”, Landtechnik, 64(1): 22-24, 2009, ISSN: 0023-8082.

VDI-RICHTLINIEN-4630: Fermentation of organic material characterization of substrate, sampling collection of material data, fermentation

tests. Dusseldorf, 92 p., 2006.

ZHONG, W.; ZHANG, Z.; QIAO, W.; FU, P.; LIU, M.: “RETRACTED: Comparison of chemical and biological pretreatment of corn straw for biogas production

by anaerobic digestion”, Renewable Energy, 36(6): 1875-1879, 2011, ISSN: 0960-1481, DOI: http://dx.doi.org/10.1016/j.renene.2010.12.020.

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