Organophosphorus pesticides degrading bacteria present in contaminated soils

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Published: Oct 13, 2016
Keywords:
soil, organophosphorus, biodegradation, metabolism, bacteria.

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Beatriz E. Jaramillo Colorado
Universidad de Cartagena
Adriana Bermúdez Tobón
Universidad de Cartagena
Irina Tirado Ballestas
Universidad de Cartagena

Abstract

The characterization of organophosphorus degrading bacteria present at three subsamples in soils from a contaminated land by pesticides, at southeast of Cartagena de Indias-Bolivar, Colombia was performed. These samples were homogenized and treated as a single sample, one part was separated for pesticide extraction and the second one was subject to monocrotophos (200 ppm) for 30 days. Bacteria capable to develop in a highly selective media with organophosphorus were isolated and biochemically identified by using BBL Crystal©kit. Bacterial growing was confirmed by means of UV-VIS spectroscopy of M9 liquid medium, and verification of pesticide bacterial degradation was perform by gas chromatography coupled flame ionization detector. According to the study at the molecular level of ribosomal gene 16S, it was determined that the three bacterial strains isolated from soil (C1, C2 and C3) belong to Enterobacteriaceae family. These species showed their ability to degrade organophosphorus pesticides.

Article Details

How to Cite
Jaramillo Colorado, B. E., Bermúdez Tobón, A., & Tirado Ballestas, I. (2016). Organophosphorus pesticides degrading bacteria present in contaminated soils. Revista Ciencias Técnicas Agropecuarias, 25(3), 13–22. Retrieved from https://revistas.unah.edu.cu/index.php/rcta/article/view/449
Issue
Vol. 25 No. 3 (2016): julio-agosto-septiembre
Section
Original Articles

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Author Biographies

Beatriz E. Jaramillo Colorado, Universidad de Cartagena

Dr.C, profesora e investigadora, Universidad de Cartagena, Campus San Pablo, Facultad de Ciencias Exactas y Naturales, Grupo de Investigaciones Agroquímicas

Adriana Bermúdez Tobón, Universidad de Cartagena

Dra., Facultad de Ciencias Exactas y Naturales

Irina Tirado Ballestas, Universidad de Cartagena

M.Sc., Facultad de Ciencias Exactas y Naturales

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