Sustainable agriculture is one of the major priorities of the 2030 Agenda, which contributes to poverty reduction through approaches in favor of the poor population, promoting the empowerment of family farming, women and youth; as well as the value chain, access to markets and social protection systems. The use of varieties of crops adapted to the conditions of the country and the analysis of production, harvesting and post-harvest processes notably help to develop precision agriculture, with a minimum cost, that guarantees the food demand of the world population.
The use and study of biostimulants and their effects is a practical alternative that contributes to the development of sustainable agricultural production systems. Biostimulants are defined as any substance or microorganism capable of increasing nutrition efficiency, tolerance to abiotic stress and the quality of crops (Du Jardin, 2015DU JARDIN, P.: “Plant biostimulants: definition, concept, main categories and regulation”, Scientia Horticulturae, 196: 3-14, 2015, ISSN: 0304-4238.). According to Drobek et al (2019)DROBEK, M.; FRĄC, M.; CYBULSKA, J.: “Plant biostimulants: importance of the quality and yield of horticultural crops and the improvement of plant tolerance to abiotic stress-a review”, Agronomy, 9(6): 335, 2019, DOI: https://dx.doi.org/10.3390/agronomy9060335. they are preparations of natural origin that support the pro-ecological cultivation of vegetables and fruits, whose effects can be multifaceted. At the National Institute of Agricultural Sciences (INCA) of the Republic of Cuba, a group of bioproducts are developed in order to increase the growth and development of crops. Among these bioproducts is QuitoMax®, a liquid biostimulant based on chitosan polymers, obtained from the chitin present in the lobster exoskeleton, with beneficial results in development, yields and anti-stress protection in several important economic crops (Morales et al., 2016MORALES, G.D.; DELL AMICO, R.J.; JEREZ, M.E.; HERNÁNDEZ, Y.D.; MARTÍN, M.R.: “Efecto del QuitoMax® en el crecimiento y rendimiento del frijol (Phaseolus vulgaris L.)”, Cultivos Tropicales, 37(1): 142-147, 2016, ISSN: 0258-5936, e-ISSN: 1819-4087.).
Horticulture is an attractive activity, mainly due to the growing demand for horticultural species with nutritional and/or medicinal value and high production volumes in small areas. Garlic (Allium sativum L.) is an annual horticultural plant of the Alliaceae family, which is used as a condiment and aromatic agent in food cooking, in addition to being used in the pharmaceutical industry, due to the chemical compounds it presents (Espinoza et al., 2010ESPINOZA, C.F.W.; RÍOS, R.E.M.; ELÍAS, P.C.C.A.: “Determinación de fenoles totales, fructanos y pungencia en seis cultivares de ajos (Allium sativum L.) en el Perú”, Revista de la Sociedad Química del Perú, 76(1): 101-109, 2010, ISSN: 1810-634X.). In Cuba, the yields of this crop are very low, despite the fact that high volumes of mineral fertilizers and pesticides are applied in its production system (Pupo et al., 2016PUPO, F.C.; GONZÁLEZ, R.G.; CARMENATE, F.O.; PEÑA, M.L.; PÉREZ, L.V.; RODRÍGUEZ, O.E.: “Respuesta del cultivo del ajo (Allium sativum L.) a la aplicación de dos bioproductos en las condiciones edafoclimáticas del centro este de la provincia Las Tunas, Cuba”, Cultivos Tropicales, 37(4): 57-66, 2016, ISSN: 0258-5936, e-ISSN: 1819-4087.).
Scientific studies related to the use of QuitoMax® or other types of biostimulants in garlic cultivation are limited (Balmori et al., 2019BALMORI, D.; DOMÍNGUEZ, C.; CARRERAS, C.; REBATOS, S.; FARÍAS, L.; IZQUIERDO, F.; BERBARA, R.; GARCÍA, A.C.: “Foliar application of humic liquid extract from vermicompost improves garlic (Allium sativum L.) production and fruit quality”, International Journal of Recycling of Organic Waste in Agriculture, 8(1): 103-112, 2019, ISSN: 2195-3228, DOI: https://dx.doi.org/10.1007/s40093-019-0279-1.10p.). Similarly, those related to the modification of the parameters of quality of the agricultural fruit are insufficient. In this sense, the aim of this work was to evaluate the quality parameters of garlic (Allium sativum L.) obtained from two Criollo clones: 'Criollo-9' and 'Criollo-Víctor' treated with concentrations of QuitoMax®.
Garlic bulbs harvested from a field experiment developed with two Criollo clones were used: 'Criollo-Víctor' and 'Criollo-9' in the Farm 'La Jaula', belonging to San José de las Lajas Municipality, Mayabeque Province, during the period 2018- 2019. The field experiment was carried out in a Sialitic Brown soil, according to the classification of Cuban soils (Hernández et al., 2015HERNÁNDEZ, J.A.; PÉREZ, J.J.M.; BOSCH, I.D.; CASTRO, S.N.: Clasificación de los suelos de Cuba 2015, Ed. Instituto Nacional de Ciencias Agrícolas e Instituto de Suelos, vol. 93, San Jose de las Lajas, Mayabeque. Cuba, 91 p., 2015, ISBN: 978-959-7023-77-7.), with pH = 7.80 and organic matter content (OM) of 5.59 %. The plantation was carried out in flower beds of (20 × 1.40 × 0.30 m) made up of soil and organic fertilizer. The cloves were planted manually with a planting density of 4 rows at 20 × 10 cm between plants.
Before planting, the cloves were soaked 24 hours in QuitoMax® solutions at concentrations of 1.0; 5.0 and 10.0 mg L-1 and in water (control treatment). At 50 days after planting (dap), foliar applications were made with the same doses of the biostimulant, keeping the group of control plants with water, for a total of four treatments for each clone. For the 'Criollo-Víctor' clone the treatments were designated as: CV-0, CV-1, CV-5 and CV-10, and for the 'Criollo-9' clone they were designated as: C9-0, C9-1, C9-5 and C9-10.
QuitoMax®, whose active ingredient is chitosan, was kindly provided by the Bioactive Products Group (GPA) of the National Institute of Agricultural Sciences (INCA). This liquid biostimulant is obtained by deacetylation of chitin extracted from lobster exoskeleton. Concentrations of 1.0, 5.0 and 10.0 mg L-1 of QuitoMax® were used, whose composition shown on the product's marketing label is presented in Table 1.
Cultural attentions were developed according to the Organoponic and Orchards Intensives Manual (INIFAT, 2010). Irrigation was carried out on alternate days with a 10 L watering can and the removal of weed plants by manual weeding. The culture was harvested 120 days after planting and 20 bulbs were selected at random from each treatment for the evaluation of external quality parameters. The raw garlic extract from each of the treatments (5 extracts per treatment) was obtained by macerating 5 g of the skinless cloves and subsequent filtration through gauze.
The caliber of bulb is a classification according to the equatorial diameter of the bulb. this work, the classification proposed by Burba (1997) was used which establishes caliber 3 (26-35 mm), caliber 4 (36-45 mm) and caliber 5 (46-55 mm).
The firmness of the bulb was performed using a CEMA C-08 Digital Penetrometer in the Laboratory of Quality and Metrology of the Faculty of Technical Sciences, UNAH. The firmness of the bulb was taken as the maximum force that the bulb supports up to the separation of the cloves, and is expressed in kg F. The ºBrix was measured using a manual refractometer placing 1 to 2 drops of the diluted garlic extract (1:10) in the prism of the refractometer, avoiding the formation of air bubbles. The measurement was made by holding the refractometer in sunlight. After each measurement (three replicates per treatment) the prism was cleaned and dried.
The pungency (pyruvic acid content) with spectrophotometer according to techniques modifications of Benklebia (2000)BENKEBLIA, N.: “Phenylalanine ammonia-lyase, peroxidase, pyruvic acid and total phenolics variations in onion bulbs during long-term storage”, LWT-Food Science and Technology, 33(2): 112-116, 2000, ISSN: 0023-6438, DOI: https://dx.doi.org/10.1006/fstl.1999.0624., Espinoza et al (2010)ESPINOZA, C.F.W.; RÍOS, R.E.M.; ELÍAS, P.C.C.A.: “Determinación de fenoles totales, fructanos y pungencia en seis cultivares de ajos (Allium sativum L.) en el Perú”, Revista de la Sociedad Química del Perú, 76(1): 101-109, 2010, ISSN: 1810-634X. and Grégrová et al (2013)GREGROVA, A.; ČÍŽKOVÁ, H.; BULANTOVA, I.; RAJCHL, A.; VOLDŘICH, M.: “Characteristics of garlic of the Czech origin”, Czech Journal of Food Sciences, 31(6): 581-588, 2013, ISSN: 1805-9317.. At 2 aliquots of the diluted garlic extract (1:10) 2 aliquots of 5 % trichloroacetic acid were added for the inactivation of the enzyme alliinase and it was left to rest for 1 hour. Then it was centrifuged for 1 min and 1 mL of 2,4-dinitrophenylhydrazine (0.125 g in 1 L of 2 mol L-1 HCl solution) was added to 1 mL of the supernatant. It was incubated in a water bath at 37°C for 15 min. Subsequently, 5 mL of NaOH 24 g L-1 were added and stirred for 5 minutes. Absorbance readings were recorded at 490 nm.
The electrical conductivity (EC) and pH (active acidity) of the diluted garlic extract (1:10) were performed by conductimetry and potentiometry respectively, according to the Cuban standard NC 39: 1999. The total measured acidity corresponds to the total acid content in the extract and was determined by acid-base volumetry, using NaOH 0.025 mol L-1 as titrant and phenolphthalein as indicator. The results were expressed as a function of the organic acids with the highest content in fruit and vegetable juices: citric, malic and tartaric acid (Domene & Segura, 2014DOMENE, M.A.; SEGURA, M.: “Parámetros de calidad interna de hortalizas y frutas en la industria agroalimentaria”, Negocios Agroalimentario Cooper Cajamar Fichas de Transferencia, 5: 1-18, 2014.), as well as pyruvic acid. The calculation was made as a function of the molar mass of each acid taking into account the volumetric law and expressed as a percentage (Table 2).
The determination of the total protein content in the extract was made by the Biuret method. To an aliquot of the diluted extract, 1 mL of distillated water and 8 mL of the Biuret reagent (mixture of copper sulfate in basic medium that forms with the peptide bonds a blue complex) were added. The dilution was shaken and around 10 min were waited for good color development. Absorbance was read at 540 nm in UV-Visible spectrophotometer (Rayleígh-1601).
The reducing carbohydrate content in the garlic extract was determined on a Rayleigh-1601 UV-visible spectrophotometer and glucose standards (0.2 to 1 mg mL-1) were used. The reducing carbohydrate content was determined using 3 mL of 3,5-dinitrosalicylic acid and 1 mL of the garlic extract, 1:10 dilution according to the methodology described by Noelting & Bernfeld (1948)NOELTING, G.; BERNFELD, P.: “Sur les enzimes amylolytiques. III. La α-amilase: dosage d’activité et controle de làbsence d’ -amilase”, Helv. Chim. Acta, 31: 286-290, 1948, DOI: https://dx.doi.org/10.1002/hlca.19480310637..
The data were processed using the Statgraph v 5.1 statistical package, a simple classification analysis of variance (ANOVA) was performed and the multiple comparison of means was achieved using the Tukey test at 95 % confidence.
The quality of the garlic bulb can be measured through various indicators such as caliber, firmness, color, pungency, total soluble solids content and phenol content (Argüello et al., 2006ARGÜELLO, J.A.; LEDESMA, A.; NÚÑEZ, S.B.; RODRÍGUEZ, C.H.; GOLDFARB, D.M. del C.: “Vermicompost effects on bulbing dynamics, nonstructural carbohydrate content, yield, and quality ofRosado Paraguayo’garlic bulbs”, HortScience, 41(3): 589-592, 2006, ISSN: 0018-5345.; Grégrová et al., 2013GREGROVA, A.; ČÍŽKOVÁ, H.; BULANTOVA, I.; RAJCHL, A.; VOLDŘICH, M.: “Characteristics of garlic of the Czech origin”, Czech Journal of Food Sciences, 31(6): 581-588, 2013, ISSN: 1805-9317.; Akan, 2019AKAN, S.: “Evaluation and Comparison of Some Parameters in Four Garlic Varieties”, Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 9(4): 1866-1875, 2019, ISSN: 2536-4618, e-ISSN: 2146-0574, DOI: https://dx.doi.org/10.21597/jist.541783.). Table 3 shows the results of the indicators of caliber, firmness and pungency of garlic bulbs from plants of the 'Criollo' clones treated with different concentrations of the non-microbial biostimulant QuitoMax®.
In none of the clones were obtained bulbs of 5 caliber (46-55 mm), this result could be justified by the fact that for the 'Criollo' clones the maximum caliber reached is 4. In the literature consulted, no reports were found of caliber (or equatorial diameter) of bulbs for 'Criollo' clones, with the exception of the 'Criollo-9' clone for which a range of values for the equatorial diameter of the bulb from 34 to 39 mm is reported (Izquierdo & Gómez, 2012IZQUIERDO, O.H.; GÓMEZ, O.: “Criollo-9, un cultivar de ajo resistente a las enfermedades fitopatógenas y elevado potencial de rendimiento”, Cultivos Tropicales, 33(2): 68-68, 2012, ISSN: 0258-5936, e-ISSN: 1819-4087.).
Table 3 shows that the influence of the use of QuitoMax® on this indicator depends on the cultivar and the concentration of the biostimulant. While for the cultivar 'Criollo-9' the major amount of bulbs with caliber 4 are founded using the QuitoMax® concentration of 5 mg L-1, for the cultivar 'Criollo-Víctor' the major amount of bulbs with caliber 4 was obtained when QuitoMax® concentrations of 1.0 and 10.0 mg L-1 are used.
It is necessary to highlight that for this last cultivar ('Criollo-Víctor') the percentage of bulbs with caliber 4 only appears in plants treated with the doses of the biostimulant QuitoMax®, this could have an important commercial and economic implication once the bulb is the commercial attribute of this crop.
The increase in the diameter of the garlic bulb in plants cultivated with biostimulants has been reported by several authors (Balmori et al., 2019BALMORI, D.; DOMÍNGUEZ, C.; CARRERAS, C.; REBATOS, S.; FARÍAS, L.; IZQUIERDO, F.; BERBARA, R.; GARCÍA, A.C.: “Foliar application of humic liquid extract from vermicompost improves garlic (Allium sativum L.) production and fruit quality”, International Journal of Recycling of Organic Waste in Agriculture, 8(1): 103-112, 2019, ISSN: 2195-3228, DOI: https://dx.doi.org/10.1007/s40093-019-0279-1.10p.; Pupo et al., 2016PUPO, F.C.; GONZÁLEZ, R.G.; CARMENATE, F.O.; PEÑA, M.L.; PÉREZ, L.V.; RODRÍGUEZ, O.E.: “Respuesta del cultivo del ajo (Allium sativum L.) a la aplicación de dos bioproductos en las condiciones edafoclimáticas del centro este de la provincia Las Tunas, Cuba”, Cultivos Tropicales, 37(4): 57-66, 2016, ISSN: 0258-5936, e-ISSN: 1819-4087.; Shafeek et al., 2015SHAFEEK, M.R.; ALI, A.H.; MAHMOUD, A.R.; HAFEZ, M.M.; RIZK, F.A.: “Improving growth and productivity of garlic plants (Allium sativum L.) as affected by the addition of organic manure and humic acid levels in sandy soil conditions.”, International journal of current microbiology and applied sciences, 4(9): 644-656, 2015, ISSN: 2319-7692, e-ISSN: 2319-7706.; Paradjikovic et al., 2014PARADJIKOVIC, N.; TKALEC, M.; VINKOVIC, S.: “Biostimulant application in transplants production of Allium sativum L. and Wild roses (Rosa canina L.).”, En: Fifth International Scientific Agricultural Symposium" Agrosym 2014", Jahorina, Bosnia and Herzegovina, October 23-26, 2014, Book of proceedings: Fifth International Scientific Agricultural Symposium" Agrosym 2014", Jahorina, Bosnia and Herzegovina, October 23-26, 2014, Ed. University of East Sarajevo, Faculty of Agriculture, Jahorina, Bosnia and Herzegovina, pp. 694-699, 2014, DOI: https://dx.doi.org/10.7251/agsy1404694p.; Denre et al., 2014DENRE, M.; GHANTI, S.; SARKAR, K.: “Effect of humic acid application on accumulation of mineral nutrition and pungency in garlic (Allium sativum L.)”, International Journal of Biotechnology and Molecular Biology Research, 5(2): 7-12, 2014, ISSN: 2141-2154, DOI: https://dx. doi.org/10.5897/IJBMBR2014.0186.; Anjum et al., 2014ANJUM, K.; AHMED, M.; BABER, K.J.; ALIZAI, A.M.; AHMED, N.; TAREEN, H.M.: “Response of garlic bulb yield to bio-stimulant (bio-cozyme) under calcareous soil”, Life Sciencie International Journal, 8(1-4): 3058-3062, 2014, ISSN: 0024-3205.; Zaki et al., 2014ZAKI, H.; TONEY, H.; ABD ELRAOUF, R.: “Response of two garlic cultivars (Allium sativum L.) to inorganic and organic fertilization”, Nature and Science, 12(10): 52-60, 2014, ISSN: 1545-0740.). Shafeek et al (2015)SHAFEEK, M.R.; ALI, A.H.; MAHMOUD, A.R.; HAFEZ, M.M.; RIZK, F.A.: “Improving growth and productivity of garlic plants (Allium sativum L.) as affected by the addition of organic manure and humic acid levels in sandy soil conditions.”, International journal of current microbiology and applied sciences, 4(9): 644-656, 2015, ISSN: 2319-7692, e-ISSN: 2319-7706., in the study of the effect of foliar application of humic acid (HA) in garlic cultivation (Chinese cv.) under field conditions, found that plants treated with the highest concentration of HA produced bulbs of higher caliber. The results of this work indicate that the use of the highest concentration of the biostimulant (QuitoMax®) does not always lead to the production of larger diameter bulbs.
The results for bulb firmness are within the range of values reported by Balmori et al (2019)BALMORI, D.; DOMÍNGUEZ, C.; CARRERAS, C.; REBATOS, S.; FARÍAS, L.; IZQUIERDO, F.; BERBARA, R.; GARCÍA, A.C.: “Foliar application of humic liquid extract from vermicompost improves garlic (Allium sativum L.) production and fruit quality”, International Journal of Recycling of Organic Waste in Agriculture, 8(1): 103-112, 2019, ISSN: 2195-3228, DOI: https://dx.doi.org/10.1007/s40093-019-0279-1.10p., who used dilutions of a humic vermicomposting extract in the cultivation of the 'Criollo-9' clone as a biostimulant. No significant differences were found between the clones or between the QuitoMax® concentrations. The latter indicates the possibility that there is no influence of this biostimulant on the number of external cataphylls (protective leaves), which influences the ease of cloves separation (López et al., 2012LOPEZ, A.M.; BURBA, J.L.; LANZAVECHIA, S.: Análisis sobre la mecanización del cultivo de ajo, Inst. Instituto Nacional de Tecnología Agropecuaria (INTA), Proyecto Ajo Estación Experimental Agropecuaria La Consulta, La Consulta, Mendoza, Argentina, 2012.).
For pungency (pyruvic acid content), which corresponds to the concentration of alliin, the precursor substrate and responsible for the smell and taste of garlic (Espinoza et al., 2010ESPINOZA, C.F.W.; RÍOS, R.E.M.; ELÍAS, P.C.C.A.: “Determinación de fenoles totales, fructanos y pungencia en seis cultivares de ajos (Allium sativum L.) en el Perú”, Revista de la Sociedad Química del Perú, 76(1): 101-109, 2010, ISSN: 1810-634X.), significant differences were found between QuitoMax® concentration only for the 'Criollo-Víctor' clone, with the highest concentrations of the biostimulant, being those with significantly higher values. These results are in correspondence with those of Denre et al (2014)DENRE, M.; GHANTI, S.; SARKAR, K.: “Effect of humic acid application on accumulation of mineral nutrition and pungency in garlic (Allium sativum L.)”, International Journal of Biotechnology and Molecular Biology Research, 5(2): 7-12, 2014, ISSN: 2141-2154, DOI: https://dx. doi.org/10.5897/IJBMBR2014.0186., researchers who in the study of the effect of different concentrations (100, 200, 300 and 400 ppm) of a commercial humic acid on the pungency of the Gargajali variety of garlic, found a significant increase in this indicator as the concentration of the biostimulant increases.
ºBrix is an indicator used in the agri-food industry to measure the approximate amount of sugars, although in reality with this scale the content of total soluble solids is determined, the most abundant being sugars and organic acids (Domene & Segura, 2014DOMENE, M.A.; SEGURA, M.: “Parámetros de calidad interna de hortalizas y frutas en la industria agroalimentaria”, Negocios Agroalimentario Cooper Cajamar Fichas de Transferencia, 5: 1-18, 2014.). In Figure 1 it is observed that for the 'Criollo-9' clone the concentration of 1 mg L-1 of QuitoMax® was significantly higher than the rest of the treatments, while for the 'Criollo-Víctor' clone there were no significant differences between treatments.
The ºBrix values found in this work are slightly lower than those reported in the literature for this cultivar from 17.2 to 21.3 ºBrix (Izquierdo & Gómez, 2012IZQUIERDO, O.H.; GÓMEZ, O.: “Criollo-9, un cultivar de ajo resistente a las enfermedades fitopatógenas y elevado potencial de rendimiento”, Cultivos Tropicales, 33(2): 68-68, 2012, ISSN: 0258-5936, e-ISSN: 1819-4087.), as well as those reported by other authors in other cultivars such as Pardo et al (2007)PARDO, J.E.; ESCRIBANO, J.; GOMEZ, R.; ALVARRUIZ, A.: “Physical-chemical and sensory quality evaluation of garlic cultivars”, Journal of Food Quality, 30(5): 609-622, 2007, ISSN: 0146-9428, DOI: https://dx.doi.org/10.1111/j.1745-4557.2007.00146.x. who report ranges from 25.1 to 29.4 ºBrix and Grégrová et al (2013)GREGROVA, A.; ČÍŽKOVÁ, H.; BULANTOVA, I.; RAJCHL, A.; VOLDŘICH, M.: “Characteristics of garlic of the Czech origin”, Czech Journal of Food Sciences, 31(6): 581-588, 2013, ISSN: 1805-9317. with reports from 31.6 to 38.7 ºBrix. However, they are within the range of values reported by Balmori et al (2019)BALMORI, D.; DOMÍNGUEZ, C.; CARRERAS, C.; REBATOS, S.; FARÍAS, L.; IZQUIERDO, F.; BERBARA, R.; GARCÍA, A.C.: “Foliar application of humic liquid extract from vermicompost improves garlic (Allium sativum L.) production and fruit quality”, International Journal of Recycling of Organic Waste in Agriculture, 8(1): 103-112, 2019, ISSN: 2195-3228, DOI: https://dx.doi.org/10.1007/s40093-019-0279-1.10p. who made foliar applications of dilutions of an extract of humic substances in the cultivar 'Criollo-9'. The results of ºBrix found in this work indicate that there should be no changes in the carbohydrate content due to the use of QuitoMax®.
For both cultivars of 'Criollo' clone, the use of QuitoMax® does not significantly modify the indicators of electrical conductivity (EC) and pH, while the titration acidity in the case of the 'Criollo-Víctor' clone is significantly modified with the concentration of the biostimulant.
Electrical conductivity is an indicative of the content of total salts, as well as acidic and basic ionizable substances. Hydronium and hydroxyl ions are the ones that most contribution to the conductivity of a solution. The values found in this work are slightly lower than those of Balmori et al (2019)BALMORI, D.; DOMÍNGUEZ, C.; CARRERAS, C.; REBATOS, S.; FARÍAS, L.; IZQUIERDO, F.; BERBARA, R.; GARCÍA, A.C.: “Foliar application of humic liquid extract from vermicompost improves garlic (Allium sativum L.) production and fruit quality”, International Journal of Recycling of Organic Waste in Agriculture, 8(1): 103-112, 2019, ISSN: 2195-3228, DOI: https://dx.doi.org/10.1007/s40093-019-0279-1.10p., authors who did find significant differences between the dilutions tested of the humic extract and the control treatment.
The pH values, indicative of the concentration of H3O+ ions in the extract, which comes from the acidic compounds present in it, are between 6.50-6.59, a range that corresponds to those reported by Pardo et al (2007)PARDO, J.E.; ESCRIBANO, J.; GOMEZ, R.; ALVARRUIZ, A.: “Physical-chemical and sensory quality evaluation of garlic cultivars”, Journal of Food Quality, 30(5): 609-622, 2007, ISSN: 0146-9428, DOI: https://dx.doi.org/10.1111/j.1745-4557.2007.00146.x. in 14 garlic cultivars: five of purple type (Moraluz, Morasol, Moratop, Mulvico and Planasa), 7 of white type (Basic, Corail, Cristo, Garcua, Ramses, Supreme and Thermidrome) and two of Chinese type (Planasa and Sprint). Other authors such as Espinoza et al (2010)ESPINOZA, C.F.W.; RÍOS, R.E.M.; ELÍAS, P.C.C.A.: “Determinación de fenoles totales, fructanos y pungencia en seis cultivares de ajos (Allium sativum L.) en el Perú”, Revista de la Sociedad Química del Perú, 76(1): 101-109, 2010, ISSN: 1810-634X. working with six garlic cultivars (Cincomesino, Barranquino precoz, Mapuri, Alfa suquia, Pata de perro and Barranquino tardío) found lower pH ranges, as did Akan (2019)AKAN, S.: “Evaluation and Comparison of Some Parameters in Four Garlic Varieties”, Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 9(4): 1866-1875, 2019, ISSN: 2536-4618, e-ISSN: 2146-0574, DOI: https://dx.doi.org/10.21597/jist.541783. who reported a range from 5.5-6.33 for four varieties of garlic: French cultivar harvested in the eastern region of France (Nice), Spanish cultivar called 'Ajo Spring Blanco', Chinese cultivar from the province of Shandong and the cultivar 'Taşköprü' from of the district with the same name located in the Black Sea Region, northern part of Turkey. According to this author, pH levels are mainly determined by irrigation, fertilization and ecological conditions. The EC and acidity values of the extract of garlic plant bulbs from the 'Criollo 9' and 'Criollo Víctor' clones treated with different concentrations of Quitomax® are presented in Table 4.
The titration acidity of the garlic extract expressed as a function of the organic acids: citric, malic, tartaric and pyruvic, is significantly different between the biostimulant concentrations only for the 'Criollo-Víctor' clone. The highest concentrations (5 and 10 mg L-1) of QuitoMax® significantly exceed the control treatment and the concentration of 1 mg L-1 of the biostimulant is significantly lower than the rest of the treatments, results that correspond to those obtained for the Pungency indicator presented previously (Table 3).
The content of organic acids influences on the taste of food, color, microbial stability and the quality of preservation, while the content of organic compounds such as proteins and carbohydrates, are related to culinary and medicinal properties of garlic (Espinoza et al., 2010ESPINOZA, C.F.W.; RÍOS, R.E.M.; ELÍAS, P.C.C.A.: “Determinación de fenoles totales, fructanos y pungencia en seis cultivares de ajos (Allium sativum L.) en el Perú”, Revista de la Sociedad Química del Perú, 76(1): 101-109, 2010, ISSN: 1810-634X.). Figure 2 shows the results for the protein content in the garlic extract of both cultivars, observing that for the cultivar 'Criollo-9' there are no significant differences between the treatments, but in the cultivar 'Criollo-Víctor' the QuitoMax® concentration of 5 mg L-1 significantly exceeds the rest of the treatments.
Increased protein concentration in plants cultivated with biostimulants has been reported by several authors (Anjum et al., 2014ANJUM, K.; AHMED, M.; BABER, K.J.; ALIZAI, A.M.; AHMED, N.; TAREEN, H.M.: “Response of garlic bulb yield to bio-stimulant (bio-cozyme) under calcareous soil”, Life Sciencie International Journal, 8(1-4): 3058-3062, 2014, ISSN: 0024-3205.; Shafeek et al., 2015SHAFEEK, M.R.; ALI, A.H.; MAHMOUD, A.R.; HAFEZ, M.M.; RIZK, F.A.: “Improving growth and productivity of garlic plants (Allium sativum L.) as affected by the addition of organic manure and humic acid levels in sandy soil conditions.”, International journal of current microbiology and applied sciences, 4(9): 644-656, 2015, ISSN: 2319-7692, e-ISSN: 2319-7706., Canellas et al., 2015CANELLAS, L.P.; OLIVARES, F.L.; AGUIAR, N.O.; JONES, D.L.; NEBBIOSO, A.; MAZZEI, P.; ALESSANDRO P: “Humic and fulvic acids as biostimulants in horticulture”, Scientia horticulturae, 196: 15-27, 2015, ISSN: 0304-4238, DOI: https://dx.doi.org/10.1016/j.scienta.2015.09.013.). In the consulted literature, few investigations were found where the content of N in the agricultural fruit of plants treated with QuitoMax® is reported. Fawzy et al (2012)FAWZY, Z.F.; EL-SHAL, Z.S.; LI, Y.S.; ZHU, O.; SAWAN, O.M.: “Response of garlic (Allium sativum, L.) plants to foliar spraying of some bio-stimulants under sandy soil condition.”, Journal of applied sciences research, (February): 770-776, 2012, ISSN: 1819-544X. report an increase in protein and nitrogen content in bulbs of garlic plants (Chinese cv.) cultivated with foliar application of Chito-Care®, a commercial product of chitosan from Egypt. Authors such as Sabreen & Mohsen (2015)SABREEN, I.K.A.; MOHSEN, A.A..: “Effect of chitosan and nitrogen rates on growth and productivity of summer squash plants”, Middle East J. Agric. Res, 4(4): 673-681, 2015, ISSN: 2077-4605. studying the effect of foliar application of chitosan concentrations at concentrations of 0.05 and 10 g L-1 in the pumpkin crop, found an increase of N content in the fruit when used the highest concentration of the biostimulant.
It is possible that the increase found in this study only for one of the concentrations in the cultivar ‘Criollo-Víctor’ is conditioned by the form and time of application of the biostimulant. In this work, an imbibition of the cloves and a single foliar application are performed at 50 dap, while for example in the work by Sabreen & Mohsen (2015)SABREEN, I.K.A.; MOHSEN, A.A..: “Effect of chitosan and nitrogen rates on growth and productivity of summer squash plants”, Middle East J. Agric. Res, 4(4): 673-681, 2015, ISSN: 2077-4605. in pumpkin, the foliar applications of chitosan were carried out three times with intervals of 10 days beginning at 25 days after planting. Another possibility could be the kind of chitosan, authors such as Costales et al (2016)COSTALES, D.; FALCÓN, R.A.; NÁPOLES, M.C.; DE WINTER, J.; GERBAUX, P.; ONDERWATER, R.C.A.; WATTIEZ, R.; CABRERA, J.C.: “Effect of chitosaccharides in nodulation and growth in vitro of inoculated soybean”, American Journal of Plant Sciences, 7: 1380-1391, 2016, DOI: https://dx.doi.org/10.4236/ajps.2016.79131. point out that the degree of deacetylation and molecular mass of chitosan influence their biological response, information that is not generally found in commercial chitosan-based biostimulants.
Figure 3 shows the results for the content of reducing carbohydrates in garlic extract from plant bulbs treated with QuitoMax®, showing significant differences between treatments. For the 'Criollo-9' cultivar, all the concentrations of the biostimulant significantly exceed the control and the concentrations of QuitoMax® 5 and 10 mg L-1 are significantly higher than 1 mg L-1. For the cultivar 'Criollo-Víctor' a similar behavior is found, all the treatments with the biostimulant concentrations are significantly higher than the control treatment with no significant differences between them.
These results do not correspond to those of ºBrix (Figure 1) where no significant modifications were found, which is logical since it is not about the content of total carbohydrates, but only the content of carbohydrates that contain a potentially free carbonyl group (monosaccharides and reducing disaccharides). Polysaccharides are not quantified by this technique because although they have the carbonyl group, it is small in the macromolecule and does not react.
In order to establish a relationship between ºBrix and carbohydrate content, it would be necessary to determine the total carbohydrate content, and especially of fructanes, such as scodorose, reserve polysaccharide and more abundant in garlic that has been related to the quality of the bulb (Argüello et al., 2006ARGÜELLO, J.A.; LEDESMA, A.; NÚÑEZ, S.B.; RODRÍGUEZ, C.H.; GOLDFARB, D.M. del C.: “Vermicompost effects on bulbing dynamics, nonstructural carbohydrate content, yield, and quality ofRosado Paraguayo’garlic bulbs”, HortScience, 41(3): 589-592, 2006, ISSN: 0018-5345.). Another possible correlation would be from the identification of the carbohydrates present in the garlic extract by techniques such as high-performance liquid chromatography (HPLC). Investigations about the influence of QuitoMax® on carbohydrate metabolism were not found. Chitosan stimulates the physiological processes in the plant and increases the size of the cells, which makes the nutrients easier to be assimilated by the plant, increasing its growth, development and yields (Rodríguez et al., 2017RODRÍGUEZ, P.A.T.; RAMÍREZ, A.M.Á.; FALCÓN, R.A.; BAUTISTA, B.S.; VENTURA, Z.E.; VALLE, F.Y.: “Effect of QuitoMax® on yield and its components of rice cultivar (Oryza sativa L.) var. INCA LP 5.”, Cultivos Tropicales, 38(4): 156-159, 2017, ISSN: 0258-5936, e-ISSN: 1819-4087.).
Even though integral mechanization of garlic cultivation is not common in Cuba, machinery such as planters or harvesters can save time and field labor. Other machinery such as sorters contributes to increasing yields and quality (López et al., 2012LOPEZ, A.M.; BURBA, J.L.; LANZAVECHIA, S.: Análisis sobre la mecanización del cultivo de ajo, Inst. Instituto Nacional de Tecnología Agropecuaria (INTA), Proyecto Ajo Estación Experimental Agropecuaria La Consulta, La Consulta, Mendoza, Argentina, 2012.). Knowledge of the physical, mechanical and chemical properties of garlic is of great importance for the mechanization of this crop and its commercial destination. The efficiency of the sheller / sorting machines depends on the caliber, firmness, number of fertile leaves, number and degree of humidity of external cataphiles. In addition, one of the most common errors in the use of planters is the poor calibration of the cloves (López et al., 2012LOPEZ, A.M.; BURBA, J.L.; LANZAVECHIA, S.: Análisis sobre la mecanización del cultivo de ajo, Inst. Instituto Nacional de Tecnología Agropecuaria (INTA), Proyecto Ajo Estación Experimental Agropecuaria La Consulta, La Consulta, Mendoza, Argentina, 2012.). The elements described above and the quantification of the chemical compounds in the different varieties of garlic are important factors when selecting the cultivars with the best chemical characteristics for the food or pharmaceutical industry (Espinoza et al., 2010ESPINOZA, C.F.W.; RÍOS, R.E.M.; ELÍAS, P.C.C.A.: “Determinación de fenoles totales, fructanos y pungencia en seis cultivares de ajos (Allium sativum L.) en el Perú”, Revista de la Sociedad Química del Perú, 76(1): 101-109, 2010, ISSN: 1810-634X.).
Several authors have justified the different values of the indicators evaluated in this study by the origin of the garlic, the type of soil where the planting was carried out, the cultural attentions provided and environmental factors. According to Akan (2019)AKAN, S.: “Evaluation and Comparison of Some Parameters in Four Garlic Varieties”, Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 9(4): 1866-1875, 2019, ISSN: 2536-4618, e-ISSN: 2146-0574, DOI: https://dx.doi.org/10.21597/jist.541783., the effect of the variety is significant in morphological indicators, but not in the case of biochemical indicators, suggesting that this latter behavior can be explained by genetic and environmental conditions. In this work, both cultivars were planted under the same experimental conditions, so the responses to the application of QuitoMax® would be conditioned by the type of cultivar.
With the use of biostimulants, not only to increase the size of the agricultural fruit is intended, but also to avoid an alteration to the detriment of the already established quality values for the fruit. In this work, there were no notable variations in garlic quality parameters for both clones, making it possible to use QuitoMax® in the production of this crop. The few modifications found in the quality indicators evaluated (size, pungency, % of organic acids, content of reducing carbohydrates and proteins) contribute to the increase in internal quality and depend on the concentration of the biostimulant and the variety.