Structural and Functional Evaluation of Humic Acids in Interaction with Toxic Metals in a Cultivar of Agricultural Interest

Liane Portuondo-Farías

Resumen

There are plants with the capacity to tolerate stress conditions due to toxic metals by developing mechanisms that allow them
to maintain homeostasis. The purpose of this paper was to verify, after the addition of humid acids, as possible stimulators of these mechanisms,
the activity of phenylalanine-ammonium-liase and nitrate reductase enzymes, content of total nitrate, proline, (as components both of
the antioxidant system of bean) as well as nitrogen, in plants subjected to stress by Pb2+. Bean seeds were planted in a typical red Ferrallitic
soil, artificially contaminated with 100 mg kg-1 of Pb2+. Fifteen days after germination, the plants were treated with humic acid solutions at
concentrations of 20, 40 and 60 mg L-1 through foliar spraying, on the first and first trifoliate leaves. Every fifteen days enzyme activities were
analyzed. Results showed a decrease of biochemical-physiological effects caused by Pb2+ in the enzymatic systems of plants treated with humic
acids, stating categorically that they may mitigate the stress provoked by metals in the phenological stages evaluated in bean crop, being 40
mg L-1 the most prominent dosage.

Palabras clave

humic substance, oxidative stress, defense mechanism

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Referencias

ALMEIDA, E.L. de; MARCOS, F.C.C.; SCHIAVINATO, M.A.; LAGÔA, A.M.M.A.; ABREU, M.F. de: “Crescimento de feijão de porco na

presença de chumbo”, Bragantia, 67(3): 569-576, 2008, ISSN: 0006-8705, DOI: 10.1590/S0006-87052008000300003.

BARROS, J.; SERRANI-YARCE, J.C.; CHEN, F.; BAXTER, D.; VENABLES, B.J.; DIXON, R.A.: “Role of bifunctional ammonia-lyase in

grass cell wall biosynthesis”, Nature Plants, 2(6): 16050, 2016, ISSN: 2055-0278, DOI: 10.1038/nplants.2016.50.

BATES, L.S.; WALDREN, R.P.; TEARE, I.D.: “Rapid determination of free proline for water-stress studies”, Plant and Soil, 39(1): 205-207,

, ISSN: 0032-079X, 1573-5036, DOI: 10.1007/BF00018060.

CORPAS, F.J.; BARROSO, J.B.: “Lead-induced stress, which triggers the production of nitric oxide (NO) and superoxide anion (O2(·-))

in Arabidopsis peroxisomes, affects catalase activity.”, Nitric Oxide : biology and chemistry, 2016, ISSN: 1089-8603, DOI: 10.1016/j.

niox.2016.12.010, Disponible en: http://europepmc.org/abstract/med/28039072, [Consulta: 23 de junio de 2017].

GARCÍA, A.C.; DE SOUZA, L.G.A.; PEREIRA, M.G.; CASTRO, R.N.; GARCÍA-MINA, J.M.; ZONTA, E.; LISBOA, F.J.G.; BERBARA,

R.L.L.: “Structure-Property-Function Relationship in Humic Substances to Explain the Biological Activity in Plants”, Scientific Reports,

: 20798, 2016, ISSN: 2045-2322, DOI: 10.1038/srep20798.

HARPER, J.E.: “Evolution of Nitrogen Oxide(s) during In Vivo Nitrate Reductase Assay of Soybean Leaves”, Plant Physiology, 68(6): 1488-

, 1981, ISSN: 0032-0889, 1532-2548, DOI: 10.1104/pp.68.6.1488.

HERNÁNDEZ, J.A.; PÉREZ, J.M.; BOSCH, D.; RIVERO, L.; CAMACHO, E.; RUÍZ, J.; SALGADO, E.J.; MARSÁN, R.; OBREGÓN, A.;

TORRES, J.M.; GONZÁLES, J.E.; ORELLANA, R.; PANEQUE, J.; RUIZ, J.M.; MESA, A.; FUENTES, E.; DURÁN, J.L.; PENA, J.;

CID, G.; PONCE DE LEÓN, D.; HERNÁNDEZ, M.; FRÓMETA, E.; FERNÁNDEZ, L.; GARCÉS, N.; MORALES, M.; SUÁREZ, E.;

MARTÍNEZ, E.: Nueva versión de clasificación genética de los suelos de Cuba, Ed. AGROINFOR, La Habana, Cuba, 64 p., 1999, ISBN:

-246-022-1.

HOWLADAR, S.M.: “A novel Moringa oleifera leaf extract can mitigate the stress effects of salinity and cadmium in bean (Phaseolus vulgaris

L.) plants”, Ecotoxicology and Environmental Safety, 100: 69-75, 2014, ISSN: 0147-6513, DOI: 10.1016/j.ecoenv.2013.11.022.

IUSS WORKING GROUP WRB: World reference base for soil resources 2006. A framework for international classification, correlation and

communication, Micheli, E. (ed.), ser. World Soil Resources Reports, no. ser. 103, Ed. Food and Agriculture Organization of the United

Nations, 2.a ed., Rome, Italy, 128 p., Primera Actualización, 2007, ISBN: 978-92-5-105511-3.

LI, Y.; ZHOU, C.; HUANG, M.; LUO, J.; HOU, X.; WU, P.; MA, X.: “Lead tolerance mechanism in Conyza canadensis: subcellular distribution,

ultrastructure, antioxidative defense system, and phytochelatins”, Journal of Plant Research, 129(2): 251-262, 2016, ISSN: 0918-9440,

-0860, DOI: 10.1007/s10265-015-0776-x.

MARTINEZ-BALMORI, D.; SPACCINI, R.; AGUIAR, N.O.; NOVOTNY, E.H.; OLIVARES, F.L.; CANELLAS, L.P.: “Molecular Characteristics

of Humic Acids Isolated from Vermicomposts and Their Relationship to Bioactivity”, Journal of Agricultural and Food Chemistry,

(47): 11412-11419, 2014, ISSN: 0021-8561, DOI: 10.1021/jf504629c.

NAKATA, H.; NAKAYAMA, S.M.M.; OROSZLANY, B.; IKENAKA, Y.; MIZUKAWA, H.; TANAKA, K.; HARUNARI, T.; TANIKAWA,

T.; DARWISH, W.S.; YOHANNES, Y.B.; SAENGTIENCHAI, A.; ISHIZUKA, M.: “Monitoring Lead (Pb) Pollution and Identifying

Pb Pollution Sources in Japan Using Stable Pb Isotope Analysis with Kidneys of Wild Rats”, International Journal of Environmental

Research and Public Health, 14(1): 56, 2017, ISSN: 1661-7827, DOI: 10.3390/ijerph14010056.

NIEUWENHUIZE, J.; POLEY-VOS, C.H.; AKKER, A.H. van den; DELFT, W. van: “Comparison of microwave and conventional extraction

techniques for the determination of metals in soil, sediment and sludge samples by atomic spectrometry”, Analyst, 116(4): 347-351, 1991,

ISSN: 1364-5528, DOI: 10.1039/AN9911600347.

SCHNEIDER, J.; BUNDSCHUH, J.; DO NASCIMENTO, C.W.A.: “Arbuscular mycorrhizal fungi-assisted phytoremediation of a lead-contaminated

site”, Science of The Total Environment, 572: 86-97, 2016, ISSN: 0048-9697, DOI: 10.1016/j.scitotenv.2016.07.185.

TAIZ, L.; ZEIGER, E.: Fisiologia Vegetal, [en línea], Ed. Artmed, 5.a ed., Porto Alegre, 918 p., 2013, ISBN: 978-85-363-2795-2, Disponible

en: https://www.saraiva.com.br/fisiologia-vegetal-5-ed-2013-4269764.html, [Consulta: 23 de junio de 2017].

VENKATACHALAM, P.; JAYALAKSHMI, N.; GEETHA, N.; SAHI, S.V.; SHARMA, N.C.; RENE, E.R.; SARKAR, S.K.; FAVAS, P.J.C.:

“Accumulation efficiency, genotoxicity and antioxidant defense mechanisms in medicinal plant Acalypha indica L. under lead stress”,

Chemosphere, 171: 544-553, 2017, ISSN: 0045-6535, DOI: 10.1016/j.chemosphere.2016.12.092.

WU, H.-H.; ZOU, Y.-N.; RAHMAN, M.M.; NI, Q.-D.; WU, Q.-S.: “Mycorrhizas alter sucrose and proline metabolism in trifoliate orange

exposed to drought stress”, Scientific Reports, 7: 42389, 2017, ISSN: 2045-2322, DOI: 10.1038/srep42389.

ZHANG, S.; ZHANG, F.; HUA, B.: “Enhancement of phenylalanine amnomia lyase, pholyphenoloxidase, and peroxidase in cucumber seedlings

by Bernisia tabaci (Gennadius)”, Agriculture Sciences in China, 7(1): 82-87, 2000, ISSN: 1671-2927.

ZHANG, X.; LIU, C.-J.: “Multifaceted Regulations of Gateway Enzyme Phenylalanine Ammonia-Lyase in the Biosynthesis of Phenylpropanoids”,

Molecular Plant, ser. Plant Metabolism and Synthetic Biology, 8(1): 17-27, 2015, ISSN: 1674-2052, DOI: 10.1016/j.molp.2014.11.001.

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